Imaging chromatin dynamics is crucial to understand genome organisation and its role in transcriptional regulation. Chromatin folding of enhancer regions are of particular interest because they can regulate gene expression over long genomic distances. What we currently know about 3D genome organisation from imaging based methods relies mainly on Fluorescence in Situ Hybridization (FISH) on fixed cells, which misses dynamic information. Recently, there has been significant progress in live cell imaging of biological processes including tools to tag genomic DNA in a live cell. The most common ones employ fluorescently tagged Cas9 and TALE proteins. However, the binding dynamics of these proteins to their target sequences are still unclear. My PhD project aims to understand firstly, the binding kinetics of Cas9 and TALE proteins to LacO arrays integrated in human cells and  compare them with the lac repressor using Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS). This will allow me, using advanced super-resolution microscopy techniques, to develop a method that will enable the visualisation of single copy loci in living cells.

Conferences I have attended: 

Biophysics of Epigenetic and Chromatin Dynamics 2018

ESRIC Interdisciplinary Consortium Meeting 2018